domed caps Search Results


domed tube caps brandtech #781340  (BrandTech)
90
BrandTech domed tube caps brandtech #781340
Domed Tube Caps Brandtech #781340, supplied by BrandTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
domed tube caps brandtech #781340 - by Bioz Stars, 2026-04
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rnase-free thin-wall tubes with domed caps 14230205  (Fisher Scientific)
90
Fisher Scientific rnase-free thin-wall tubes with domed caps 14230205
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
Rnase Free Thin Wall Tubes With Domed Caps 14230205, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnase-free thin-wall tubes with domed caps 14230205/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
rnase-free thin-wall tubes with domed caps 14230205 - by Bioz Stars, 2026-04
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thin-walled polymerase chain reaction tube with dome caps  (Fisher Scientific)
90
Fisher Scientific thin-walled polymerase chain reaction tube with dome caps
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
Thin Walled Polymerase Chain Reaction Tube With Dome Caps, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thin-walled polymerase chain reaction tube with dome caps/product/Fisher Scientific
Average 90 stars, based on 1 article reviews
thin-walled polymerase chain reaction tube with dome caps - by Bioz Stars, 2026-04
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8-strip domed tube caps  (BrandTech)
90
BrandTech 8-strip domed tube caps
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
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ethylene vinyl acetate dome shaped caps soft-try  (Ultradent Products Inc)
90
Ultradent Products Inc ethylene vinyl acetate dome shaped caps soft-try
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
Ethylene Vinyl Acetate Dome Shaped Caps Soft Try, supplied by Ultradent Products Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethylene vinyl acetate dome shaped caps soft-try - by Bioz Stars, 2026-04
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thin-walled rnase-free pcr tube  (Fisher Scientific)
90
Fisher Scientific thin-walled rnase-free pcr tube
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
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domed pcr caps  (Ultident Scientific)
90
Ultident Scientific domed pcr caps
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
Domed Pcr Caps, supplied by Ultident Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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8-strip pcr tube caps with dome top, natural  (Watson Bio Lab)
90
Watson Bio Lab 8-strip pcr tube caps with dome top, natural
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
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8-strip caps, dome top  (E&K Scientific)
90
E&K Scientific 8-strip caps, dome top
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
8 Strip Caps, Dome Top, supplied by E&K Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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custom-made dome shaped caps  (Ultradent Products Inc)
90
Ultradent Products Inc custom-made dome shaped caps
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
Custom Made Dome Shaped Caps, supplied by Ultradent Products Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr tubes domed caps  (Optika Srl)
90
Optika Srl pcr tubes domed caps
Representative results from the experiments on <t>PMCAb,</t> dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84
Pcr Tubes Domed Caps, supplied by Optika Srl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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8 well stripcap dome caps  (Genesee Scientific)
N/A
Genesee Scientific 22 170 8 Strip Dome Caps Natural Fits Genesee 0 2ml Tubes Plate Bag of 125 Strip Caps UnitFits Genesee 0 2ml Tubes Plate
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Representative results from the experiments on PMCAb, dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Methods of Protein Misfolding Cyclic Amplification

doi: 10.1007/978-1-4939-7244-9_13

Figure Lengend Snippet: Representative results from the experiments on PMCAb, dgPMCAb, and PMCAb in an RNA-depleted environment and an estimation of amplification rate. Each reaction was done in the presence of three PTFE spheres. The sonication protocol consisted of 30 s sonication pulses at 50% power performed every 30 min during 24 h. (a) PMCAb and dgPMCAb reactions were seeded with 10−2 dilution of brain material from the first passage of a synthetic strain LOTSS [22]. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. In the first passage, brain-derived LOTSS represents a mixture of authentic PrPSc and atypical PrPres, which are selectively amplified in PMCAb (upper panel) and dgPMCAb (lower panel), respectively. Amplification of PrPSc was visualized with 3F4 antibody, while atypical PrPres was detected with SAF-84. (b) PMCAb in an RNA-depleted environment illustrates RNA dependency of amplification of 263 K scrapie material. The reactions were seeded with 10−3 diluted brain material from terminally ill hamsters. Ten microliters of reaction products from a completed round were used to seed 90 μl of the subsequent round. Western blot was stained with SAF-84. (c). Estimation of an amplification rate for LOTSS. PMCAb reactions were seeded with 10−4 diluted brain material from the second passage of LOTSS. For the dilution factor ×30, 10 μl of a completed reaction were mixed with 20 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factor ×100, 10 μl of a completed reaction were mixed with 90 μl of conversion buffer, and 10 μl of this dilution was used to seed subsequent PMCAb rounds. For the dilution factors ×300 and ×1000, 10 μl of a completed reaction was diluted threefold and tenfold, respectively, into conversion buffer and then diluted further by mixing 10 μl with 90 μl of conversion buffer each, and 10 μl out of these dilutions were used to seed subsequent PMCAb rounds. An amplification rate of LOTTS was estimated to be 300-fold per PMCAb round. An increase in intensity of the signal from round to round illustrates the ability of synthetic strains to quickly adapt to an PMCAb environment and increase its amplification efficiency as previously described [23]. Western blot was stained with SAF-84

Article Snippet: Protein Misfolding Cyclic Amplification with Beads (PMCAb) RNase-free 0.2 ml thin-wall tubes with domed caps (14230205, Fisher Scientific).

Techniques: Amplification, Sonication, Derivative Assay, Western Blot, Staining

Schematic representation of a PMCAb experiment for estimating a strain-specific amplification rate. Several serial PMCAb reactions with incrementing dilution factors between rounds are initiated using the same scrapie material. In each individual serial PMCAb reaction, the dilution factor between rounds is kept constant. The highest dilution factor at which PrPSc amplification fully compensates the effect of dilution determines the amplification rate

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Methods of Protein Misfolding Cyclic Amplification

doi: 10.1007/978-1-4939-7244-9_13

Figure Lengend Snippet: Schematic representation of a PMCAb experiment for estimating a strain-specific amplification rate. Several serial PMCAb reactions with incrementing dilution factors between rounds are initiated using the same scrapie material. In each individual serial PMCAb reaction, the dilution factor between rounds is kept constant. The highest dilution factor at which PrPSc amplification fully compensates the effect of dilution determines the amplification rate

Article Snippet: Protein Misfolding Cyclic Amplification with Beads (PMCAb) RNase-free 0.2 ml thin-wall tubes with domed caps (14230205, Fisher Scientific).

Techniques: Amplification

A floating rack for holding PMCAb tubes in the microplate horn is made from a 6 mm extra firm foam sheet available in craft stores

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Methods of Protein Misfolding Cyclic Amplification

doi: 10.1007/978-1-4939-7244-9_13

Figure Lengend Snippet: A floating rack for holding PMCAb tubes in the microplate horn is made from a 6 mm extra firm foam sheet available in craft stores

Article Snippet: Protein Misfolding Cyclic Amplification with Beads (PMCAb) RNase-free 0.2 ml thin-wall tubes with domed caps (14230205, Fisher Scientific).

Techniques: CRAfT Assay